Posted on

The impact of the Gravity Prescription on the future of space settlement

Artist rendering of a family living in a rotating free-space settlement based on the Kalpana Two design, with a length of 110m and diameter of 125m. Credits: Bryan Versteeg / Spacehabs.com

This post summarizes my upcoming talk for the Living in Space Track at ISDC 2024 taking place in Los Angeles May 23 – 26. The presentation is a distillation of several posts on the Gravity Prescription about which I’ve written over the years.

Lets start with a couple of basic definitions. First, what exactly is a space settlement? The National Space Society defined the term with much detail in an explainer by Dale L. Skran back in 2019. I’ve extracted this excerpt with bolded emphasis added:

Space Settlement is defined as: ​

​“… a habitation in space or on a celestial body where families live on a permanent basis, and that engages in commercial activity which enables the settlement to grow over time, with the goal of becoming economically and biologically self-sustaining …”

​The point here is that people will want to have children wherever their families put down roots in space communities. Yes, a “settlement” could be permanent and perhaps inhabited by adults that live out the rest of there lives there, such as in a retirement community. But these are not biologically self-sustaining in the sense that settlers have offspring that are conceived, born and raised there living out healthy lives over multiple generations.

Next we should explain what is meant by the Gravity Prescription (GRx). First coined by Dr. Jim Logan, the term refers to the minimum “dosing” of gravity (level and duration of exposure) to enable healthy conception, gestation, birth and normal, viable development to adulthood as a human being…over multiple generations. It should be noted that the GRx can be broken down into at least three components: the levels needed for pregnancy (conception through birth), early child development, and adulthood. The focus of this discussion is primarily on the GRx for reproduction.

We should also posit some basic assumptions. First, with the exception of the GRx, all challenges expected for establishment of deep space settlements can be solved with engineering solutions (e.g. radiation protection, life support, power generation, etc…)​. The one factor that cannot be easily changed impacting human physiology after millions of year of evolution on Earth is gravity. We may find it difficult or even impossible to stay “healthy enough” under hypogravity conditions on the Moon or Mars, assuming all other human factors are dealt with in habitat design.

Lets dive into what we know and don’t know about the GRx. Several decades of human spaceflight have produced an abundance of data on the deleterious effects of microgravity on human physiology, not the least of which are serious reduction in bone and muscle mass, ocular changes, and weakening of the immune system – there are many more. So we know microgravity is not good for human health after long stays. Clearly, having babies under these conditions would not be ethical or conducive for long term settlement.

The first studies carried out on mammalian reproduction in microgravity took place in the early 1990s aboard the Space Shuttle in a couple of experiments on STS-66 and STS-70. 10 pregnant rats were launched at midpregnancy (9 days and 11 days, respectively) on each flight and landed close to the (22 day) term. The rat pups were born 2 days after landing and histology of their brain tissue found spaceflight induced abnormalities in brain development in 70% of the offspring.

It was not until 2017 that the first mammalian study of rodents with artificial gravity was performed on the ISS. Although not focused on reproduction, the Japan Aerospace Exploration Agency (JAXA) performed a mouse experiment in their Multiple Artificial-gravity Research System (MARS) centrifuge comparing the impact of microgravity to 1g of spin gravity. ​The results provided the first experimental evidence that mice exposed to 1g of artificial gravity maintained the same bone density and muscle weight as mice in a ground control group while those in microgravity had significant reductions.

Diagram depicting an overview of the first JAXA Mouse Project in the MARS centrifuge with photos of the experiment on the ISS. Credits: Dai Shiba et al. / Nature. http://creativecommons.org/licenses/by/4.0/

In 2019 JAXA carried out a similar study in the MARS centrifuge adding lunar gravity levels to the mix. This study found that there were some benefits to the mice exposed to 1/6g in that Moon gravity helped mitigate muscle atrophy, but it did not prevent changes in muscle fiber or gene expression​.

Just last year, a team led by Dr. Mary Bouxsein at Harvard Medical School conducted another adult mouse study on the MARS centrifuge comparing microgravity, .33g, .67g and 1g. They found that hind quarter muscle strength increased commensurate with the level artificial gravity concluding, not surprisingly, that spaceflight induced atrophy can be mitigated with centrifucation. The results were reported at the American Society for Gravitational and Space Research last November.​

Returning to mammalian reproduction in space, an interesting result was reported last year in the journal Cell from an experiment by Japanese scientists at the University of Yamanashi carried out on the ISS in 2019. The team, headed up by Teruhiko Wakayama, devised a way to freeze mouse embryos post conception and launch them into space where they were thawed by astronauts and allowed to develop in microgravity. Control samples were cultured in 1g artificial gravity on the ISS and Earth normal gravity on the ground. The mouse embryos developed into blastocysts and showed evidence of cell differentiation/gene expression in microgravity after 4 days​. The researchers claimed that the results indicated that “Mammals can thrive in space”. This conclusion really can’t be substantiated without further research.

Which brings us to several unknowns about reproduction in space. SSP has explored this topic in depth through an interview with Alex Layendecker, Director of the Astrosexological Research Institute. Yet to be studied in depth is (a) conception, including proper transport of a zygote through the fallopian tube to implantation in the uterus. Less gravity may increase the likelihood of ectopic pregnancy which is fatal for the fetus and could endanger the life of the mother; (b) full gestation through all stages of embryo development to birth​; and (c) early child development and maturation to adulthood in hypogravity​. All these stages of mammalian reproduction need to be validated through ethical clinical studies on rodents progressing to higher primate animal models before humans can know if having children in lower gravity conditions on the Moon or Mars will be healthy and sustainable over multiple generations.

AI generated image of an expectant mother with her developing fetus in Earth orbit after mammalian reproduction has been validated via higher animal models through all stages of pregnancy for a safe level of gravity. An appropriate level of radiation shielding would also be required and is not shown in this illustration. Credit: DALL-E-3

Some space advocates for communities on the Moon or Mars have downplayed the importance of determining the GRx for reproduction with the logic that a fetus in a woman’s uterus on Earth is in neutral buoyancy and thus is essentially weightless. Therefore, why does gravity matter? ​ I discussed this question with Dr. Layendecker and he had the following observations paraphrased here: True, gravity may have less of an impact in the first trimester. But on the cellular level, cytoskeletal development and proper formation/organization of cells may be impacted from conception to birth​. Gravity helps orient the baby for delivery in the last trimester​ and keeps the mother’s uterine muscles strong for contractions/movement of the baby through the birth canal​. There are many unknowns on what level of gravity is sufficient for normal development from conception to adulthood.

Why does all this matter? Ethically determining the right level of gravity for healthy reproduction and child development will inform where families can safely settle space​. The available surface gravities of bodies where we can establish communities in space cluster near Earth, Mars and Moon levels​. These are our only GRx options ​on solar system bodies.

Gravity level clustering of solar system bodies available for space settlement. Credit: Joe Carroll

The problem is that we don’t yet know whether we can remain healthy enough on bodies with gravity equivalent to that on the Moon or Mars, so we can’t select realistic human destinations or formulate detailed plans until we acquire this knowledge​. Of course we can always build rotating settlements in free space with artificial gravity equivalent to that on Earth. Understanding the importance of the GRx and determining its value could change the strategy of space development in terms of both engineering and policy decisions. The longer we delay, the higher the opportunity costs in terms of lost time from failure to act​.

What are these opportunity cost lost opportunities​? Clearly, at the top of Elon Musk’s list is “Plan B” for humanity, i.e. a second home in case of cataclysmic disaster such as climate change, nuclear war, etc. This drives his sense of urgency. From Gerard K. O’Neill’s vision in The High Frontier, virtually unlimited resources in space could end hunger and poverty, provide high quality living space for rapidly growing populations​, achieve population control without war, famine, or dictatorships​. And finally, increase freedom and the range of options for all people​.

If humans can’t have babies in less than Earth’s gravity then the Moon and Mars may be a bust for long term (biologically sustainable) space settlement.​ There will be no biologically sustainable cities with millions of people on other worlds unless they can raise families there​.

Spin gravity rotating space settlements providing 1g artificial gravity may be the only alternative​. If Elon Musk knew that the people he wants to send to Mars can’t have children there, would he change his plans for a self-sustaining colony on that planet?​ Having and raising children is obviously important to him. As Walter Isaacson wrote in his recent biography of Musk, “He feared that declining birthrates were a threat to the long-term survival of human consciousness.”

So how could he determine the GRx quickly? One solution would be to fund a partial gravity facility in low Earth orbit to run ethical experiments on mammalian reproduction in hypogravity. Joe Carroll has been refining a proposal for such a facility, a dual dumbbell Moon/Mars low gravity laboratory which SSP has covered, that could also be marketed as a tourist destination. Spinning at 1.5 rpm, the station would be constructed from a combination of Starship payload-sized habitats tethered by airbeams allowing shirt sleeve access to different gravity levels​. Visitors would be ferried to the facility in Dragon capsules and could experience 3 gravity levels with various tourist attractions​. The concept would be faster, cheaper, safer and better than establishing equivalent bases on the Moon or Mars to quickly learn about the GRx​. The facility would be tended by crews at both ends that live & collect health data for up to a year or more​. And of course, ethical experiments on the GRx for mammalian reproduction would be carried out, first on rodents and then progressing to higher primates if successful.

Left: Conceptual illustration depicting a LEO Moon-Mars dumbbell partial gravity facility constructed from Starship payload-sized habitats tethered by airbeams and serviced by Dragon capsules. Rectangular solar arrays deploy by hanging at either end as spin is initiated via thrusters at Mars module. Center: Image of an inflated airbeam demonstration. Right: diagram of an airbeam stowed for transport and after deployment. Credit: Joe Carroll

What if these experiments determine that having children in lower gravity is not possible and our only path forward are free-space rotating settlements? Physics and human physiology require that they be large enough for settlers to tolerate a 1g spin rate to prevent disorientation. As originally envisioned by O’Neill, the diameter of his Island One space settlement would be about 500 meters.

Conceptual illustration of an Island One space settlement. The living space sphere is sized at about 500m in diameter. Credits: Rick Guidice / NASA

As originally proposed, these settlements would be located outside the Earth’s magnetic field at the L5 Earth-Moon Lagrange Point necessitating that they be shielded with enormous amounts of lunar regolith to protect occupants from radiation. Their construction requires significant technology development and infrastructure (e.g. mass drivers on the Moon, automated assembly in space, advances in robotics, power sources, etc…)​. Much of this will eventually be done anyway as space development progresses…however, knowing the GRx (if it is equal to 1g) may foster a sense of urgency​.

Some may take the alternative viewpoint that if we know that Earth’s gravity works just fine we could proceed directly to free-space settlements if we could overcome the mass problem. This is the approach Al Globus and Tom Marotta took in their book The High Frontier: An Easier Way with Kalpana One​, a 450m diameter cylindrical rotating free-space settlement located in equatorial low Earth orbit (ELEO) protected by our planet’s magnetic field, thereby reducing the mass significantly because there would be far less need for heavy radiation shielding.

Artist impression of Kalpana One rotating free-space settlement located in equatorial low Earth orbit. Credits: Bryan Versteeg / Spacehabs.com

But there may be an even easier way. Kasper Kubica has proposed a 10 year roadmap to the $10M condo in ELEO based on Kalpana Two, a scaled down version of the orbital settlement described by Al Globus in a 2017 Space Review article.

Artist rendering of the inside of a rotating free-space settlement based on the Kalpana Two design, with a length of 110m and diameter of 125m. Credits: Bryan Versteeg / Spacehabs.com

Even though these communities would be lower mass, they will still require significant increases in launch rates to place the needed materials in LEO, especially near the equator​. Offshore spaceports, like those under development by The Spaceport Company, could play a significant role​ in this infrastructure. Legislation providing financial incentives to municipalities to build spaceports would be helpful, such as The Secure U.S. Leadership in Space Act of 2024 introduced in Congress last month. The new law (not yet taken up in the Senate) would amend the IRS Code to allow spaceports to issue tax-exempt Muni bonds for infrastructure improvements.

Wouldn’t orbital debris present a hazard for settlements in ELEO?​ Definitely yes, and the National Space Society is shaping policy in this area. The best approach is to emphasize “light touch” regulatory reform on salvage rights, with protection and indemnity of the space industry to encourage recycling and debris removal.​ Joe Carroll has suggested a market-based approach that would impose parking fees for high value orbits, which would fund a bounty system for debris removal. This system would incentivize companies like CisLunar Industries, Neumann Space and Benchmark Space Systems, firms that are developing space-based processes to recycle orbital debris into useful commodities such as fuel and structural components.

Further down the road in technology development and deeper into space, advances in artificial intelligence and robotics will enable autonomous conversion of asteroids into rotating space settlements, as described by David Jensen in a paper uploaded to arXiv last year.​ This approach significantly reduces launch costs by leveraging in situ resource utilization. Initially, small numbers of “seed” tool maker robots are launched to a target asteroid​ along with supplemental “vitamins” of components like microprocessors that cannot be easily fabricated until technology progresses, to complete the machines. These robotic replicators use asteroid materials to make copies of themselves and other structural materials eventually building out a rotating space settlement. As the technology improves, the machines eventually become fully self-replicating, no longer requiring supplemental shipments from Earth.

Artist impression of a rotating space settlement constructed from asteroid materials. Credits: Bryan Versteeg, spacehabs.com

Leveraging AI to enable robots to build space settlements removes humans from the loop initially, eliminating risk to their health from exposure to radiation and microgravity​. Send it the robot home builders – families then safely move in later. There are virtually unlimited supplies in the asteroid belt to provide feedstock to construct thousands of such communities.

Artist impression of the interior of Stanford Torus free-space settlement. Advances in artificial intelligence and robotics will enable autonomous self replicating machines that could build thousands of such communities from asteroid material. Credits: Don Davis / NASA

If rotating space settlements with Earth-normal gravity become the preferred choice for off-Earth communities, where would be the best location, the prime real estate of the solar system? Jim Logan has identified the perfect place with his Essential Seven Settlement Criteria.

  • Low Delta-V​ – enabling easy access with a minimum of energy
  • Lots of RESOURCES​ … obviously!
  • Little or No GRAVITY WELL​ – half way to anywhere in the solar system
  • At or Near Earth Normal GRAVITY for​
    People, Plants and Animals ​- like what evolved on Earth
  • Natural Passive 24/7 RADIATION Protection​ – for healthy living
  • Permit Large Redundant Ecosystem(s)​ – for sustenance and life support
  • Staging Area for Exploration and Expansion​
    (including frequent, recurrent launch windows)​

Using this criteria, Logan identified Deimos, the outermost moon of Mars, as the ideal location. As discussed above, AI and robotic mining technology improvements will enable autonomous boring machines to drill a 15km long core through this body with a diameter around 500 meters – sized for an Island One space settlement to fit perfectly.

Conceptual illustration of a 500 meter wide by 15km long core bored through Deimos. Credit: Jim Logan

In fact, 11 Island One space colonies (minus the mirrors) strung end to end through this tunnel would provide sea level radiation protection and Earth normal artificial gravity for thousands of healthy settlers.

Left: Artist impression of an Island One space settlement. Credits: Rick Guidice / NASA. Right: To scale depiction of 11 Island One space settlements strung end-to-end in a cored out tunnel through Deimos providing sea level radiation protection and Earth normal artificial gravity. Credit: Jim Logan

In conclusion, the GRx for reproduction will inform where biologically self-sustaining healthy communities can be established in space. If we find that the GRx is equal to Earth’s normal level, free-space settlements with artificial gravity will be the safest and healthiness solution for humans to live and thrive throughout the solar system. The sooner we determined the GRx the better, for current plans for settling the Moon or Mars may need to be altered to consider rotating space colonies, which will require significant infrastructure development and regulatory reform​. Alternatively, since we know Earth’s gravity works just fine, we may choose to skip determination of the GRx and start small with Kalpana in low Earth orbit. Eventually, artificial intelligence will enable safe, autonomous self-assembly of space settlements from asteroids. The interior of Deimos would be the perfect place to build safe, healthy and biologically self-sustaining space settlements.

Posted on

JAXA’s Lunar Farming Concept Study

Cutaway illustration depicting a sublunar farm covered by regolith, providing food and augmenting life support for a settlement on the Moon. Credits: Microsoft Image Creator

The Japan Aerospace Exploration Agency (JAXA) published a report last November summarizing the findings of its Lunar Farming Concept Study Working Group. JAXA’s team, composed of professionals in universities and private experts, assumes that humans will eventually establish permanent communities on the Moon and conducted the study using cutting-edge agriculture science and biotechnology to design a plant factory that would provide nutritional sustenance and oxygen in a life support system for a lunar settlement.

The working group was composed of four subgroups: cultivation, unmanned technology, recycling, and overall system design. The cultivation subgroup focused on the farm’s environmental controls including light levels (provided by LEDs), irrigation and atmospheric conditions tailored to each crop type. The unmanned technology team dealt with robotic maintenance of the plant factory environment including autonomous monitoring, sowing, cultivating and harvesting. The recycling group ensured soil improvement and reuse of limited resources, inedible scraps and waste material. Finally, the overall system subgroup studied the farm as a whole taking into account each plant species.

The scale of the lunar colony in the study was spit into two scenarios. An initial settlement in the near future with a 6 person crew followed by a larger scale permanent community at a later date with 100 people. The objective was to define a scalable cultivation system that would provide energy and nutritional requirements for settlers without resupply from Earth. The design would leverage recycling to fullest extent possible, minimize the use of materials sourced on the Moon such as water and oxygen from the polar regions, and reduce supplies imported from Earth, realizing that the system would not be 100% closed. LED lighting was utilized to optimize wavelength for chlorophyll absorption as well as diurnal growth cycles during the 14 day lunar night, being necessary for crop illumination in an underground farming community protected from radiation by thick layers of regolith. Nuclear power was considered as a power source.

An important finding of the study leveraged a metric called the Equivalent Systems Mass (ESM), to evaluate the life support systems of the different lunar farm designs explored by the team. ESM is a mathematical formula used to perform trade studies to determine which options have the lowest launch cost and is calculated from the system variables mass, volume, power, cooling, and crew working hours. When comparing the ESM of several biomass production systems it was found that the mass of the system could be minimized by appropriate sizing of crop cultivation shelves and increased space utilization efficiency. It was shown that over a 10 year period an optimized design for a lunar farm would not have to be replenished with food from the Earth when building materials, water and oxygen were supplemented by sources on the Moon and nuclear power was assumed as a power source.

The JAXA study adds to the space farming body of knowledge needed for establishing life support systems for space settlement.

Posted on

Sex in space and its implications for space tourism and settlement

AI generated image of an amorous couple embracing in a space tourist destination. Credits: DALL-E

Last April, an international team of researchers published a green paper to solicit public consultation on the urgent need for dialogue concerning uncontrolled human conception which will be problematic for space tourism when it takes off in the near future.   A coauthor on the paper, Alex Layendecker of the Florida based Astrosexological Research Institute (ASRI) studied the subject for his PhD thesis. Layendecker gave a talk at ISDC 2023 entitled Sex in Space in the Era of Space Tourism in which he emphasized the huge knowledge gap we have on mammalian conception, gestation and birth in the high radiation and lower gravity environments of outer space.  Since humans evolved for millions of years in Earth’s gravity protected from radiation by our planet’s magnetic field and atmosphere, there is a significant risk of developmental abnormalities in offspring which could result in legal liability and potential impacts on commerce if conception occurs in space without consideration of the potential hazards.  After his talk, I discussed these matters and the implications for space settlement with Alex who agreed to continue our discussion in an interview by email for this post.

SSP: Alex, it was a pleasure meeting you at ISDC and thank you for taking the time to answer my questions on this important topic.  The green paper is attempting to foster discussion from relevant stakeholders on addressing “uncontrolled human conception”.  Uncontrolled is defined in the paper as “…without societal approval for human conception – i.e. without regulatory approval from relevant bodies representing a broad societal consensus.” I am not aware of any regulatory authority on these matters at this time and there will likely be considerable challenges to obtain consensus across the space community before tourism becomes mainstream. The intent of the paper appears to be to help develop a framework for regulations (or guidelines) before space tourism takes off. Given how long it takes for regulations to be implemented and the challenges of international consensus, will there be enough time to implement sufficient controls before conception happens in space?

AL:  Great question – short answer up front, no, I don’t believe any “controls” will be implemented before the first incidence of human conception in space, given the timelines we’re currently looking at.  As you mentioned, regulations can take a long time to come into effect and you need to have a basis for establishing regulations/law – space law itself is still being developed.  Our knowledge of reproduction in space is minimal at this stage, certainly not at the level it needs to be at this point of history.  We’re also in virtually unexplored territory when it comes to mass space tourism – there have been space tourists in the past, Dennis Tito being the first “official” space tourist in history over 20 years ago – but all previous individuals that went into space for tourism purposes have done so while integrated into the crew, typically with very little privacy and a considerable amount of training.  With mass access to space, we’ll soon have groups of individuals going up solely for vacation/leisure purposes, and you can be assured some of them will be engaging in sexual activity.  While it would be absurd to try to implement or enforce laws preventing sexual activity in those environments, the dangers associated with potential conception still exist.  What is critically needed at this point is a better collective understanding of those dangers, their mitigation, and for space companies to be able to provide those paying customers with enough information that informed consent can be established – space is inherently dangerous already, and people launching into space are briefed on that.  They will need to be briefed on the dangers associated with conception in space as well, which could not only potentially threaten the life of the baby but also that of the mother, depending on the times and distances involved.

SSP: Will this be a government effort (since a green paper typically implies government sponsorship) or a self-imposed industry-wide trade association consensus approach like CONFERS? Or a combination?

AL: I think in the immediate sense, there will need to be a self-imposed industry consensus on establishing informed consent among space tourism customers. Sex and potential conception in space is currently a blind spot for would-be space tourism companies, because up to this point many of them haven’t considered the dangers it could pose to their customers, and corporate liability here is also an issue. It’s their responsibility to keep their passengers safe, and to inform them of any dangers to the max extent possible. I don’t necessarily see governments being able to implement or enforce any regulations in this regard, because regulating people doing what they want with their own bodies in the privacy of their own bedrooms typically doesn’t fare well over the long term. Where governments may get involved is if any medical situation develops to the point of needing rapid rescue, but Space Rescue capabilities is another topic.

SSP: Space tourism is likely to attract thrill seekers and risk-takers who are likely to have rebellious personalities with a reluctance to follow rules and regulations, let alone respect for societal norms. If this is the case, will pre-flight consultations on the risks of uncontrolled conception and legal waivers be sufficient to prevent risky behavior? Can the effectiveness of this approach be tested prior to implementation?

AL: Prevent risky behavior? Absolutely not. As you point out, these are folks who are intentionally undertaking an enormously risky endeavor in flying to space already, and at least in the early years, will be primarily comprised of your limits-pushing, boundary-breaking types. So they’re already about risk as individuals. However, legal waivers will of course be part of the whole operation, likely to include waivers around the risks of conception. Waivers or not, people are still going to engage in sex in space, and relatively soon, and if the individuals in question are capable of conception, the act itself brings that risk. Not to mention that there are individuals out there who will be vying for the title of “first couple to officially have sex in space,” despite speculation over the years that it could have occurred in the past. To be part of the first publicly declared coupling in outer space will land their names in history books. Now, there will be individuals who decide that they don’t want to deal with those risks after a thorough briefing on the potential dangers, but not everyone – probably not even a majority, knowing humans – will be deterred.

SSP: The paper highlights concerns about pregnancy in higher radiation and microgravity environments. From a space settlement perspective, radiation is less of a problem as there are engineering solutions (i.e. provision for adequate shielding) to address that issue. The bigger challenge will be pregnancies in microgravity, or in lower gravity on the Moon and Mars. The physiology of human fetus development in less than 1g is a big unknown. Some space advocates such as Robert Zubrin brush this off with the logic that a fetus in vivo on Earth is developing in essentially neutral buoyancy, and is therefore weightless anyway, so gestation in less than 1g probably won’t matter. Setting aside the issues associated with conception in lower gravity, if a woman can become pregnant in space, do you think this logic may be true for gestation or are there scientific studies and/or physiological arguments on the importance of Earth’s gravity in fetal development that refute this position?

AL: I’ve heard the neutral buoyancy argument before but it doesn’t address all the issues by a long shot. There is more neutral buoyancy during the first trimester of gestation but in the second and third gravity is very important, even just logistically speaking. Gravity helps the baby orient properly for delivery, and helps keep the mother’s uterine muscles strong enough to provide the necessary level of contractions to safely move the baby through the birth canal. On a more cellular level, cytoskeletal development is impacted by gravity, so even proper formation and organization of cells can be affected by microgravity throughout the span of gestation, from conception to birth. Gravity has a huge impact on postnatal development as well – in the small handful of NASA experiments we’ve conducted using mammalian young (baby rat and mouse pups), there were significant fatality rates among younger/less developed pups against ground control groups when exposed to microgravity during key postnatal phases. The youngest pups (5 days old) suffered a 90% mortality rate, and any of the survivors had significant developmental issues. So gravity is crucial not just to fetal development but to newborns and children as well, that much is evident from the data we do have.

SSP: Following up on your response, the Moon/Mars settlement advocates will say partial gravity levels on these worlds may be sufficiently higher than in microgravity to address the issues you mentioned – baby orientation, cytoskeletal development, cellular formation/organization, postnatal development – and a full 1g may not be needed for healthy reproduction.  The mammalian studies you mentioned with detrimental postnatal development were in microgravity.   We now have a data point at the lunar gravity level from JAXA with their long awaited results of a 2019 study on postnatal mice subjected to 1/6g partial gravity in a paper in Nature that was published last April. The good news is that 1/6g partial gravity prevents muscle atrophy in mice. The downside is that this level of artificial gravity cannot prevent changes in muscle fiber (myofiber) and gene modification induced by microgravity. There appears to be a threshold between 1/6g and Earth-normal gravity, yet to be determined, for skeletal muscle adaptation.  Have you seen these results, can you comment on them and do you think they may rule out mammalian postnatal development in lunar gravity?  

AL: With regard to the JAXA study, I think I’ve seen a short summary of preliminary results but haven’t gotten to read the full study yet. What I will comment for now is that there’s at least some promise in those results from a thousand foot view. While we still need to determine/set parameters for what we as a society/species consider medically/ethically acceptable for level of impact (obviously there was gene modification in the JAXA mice), there are clearly still some benefits to even lower levels of gravity.

SSP: With respect to risk mitigation and the paper’s recommended area of research: “Consolidation of existing knowledge about the early stages of human (and mammalian) reproduction in space environments and consideration of the ensuing risks to human progeny”, SSP has covered off-Earth reproduction and highlighted the need for ethical clinical studies in LEO to determine the gravity prescription (GRx) for mammalian (and eventually human) procreation.  During our personal discussions at ISDC, you mentioned ASRI’s plans for such studies in space.  Can you elaborate on your vision for mammalian reproduction studies in variable gravity?  What would be your experimental design and proposed timeline?

AL:  Well, with regard to timelines, humanity as a whole is already behind, so we’ll need to move as quickly as we possibly can while still upholding safe medical and ethical standards.  We’re approaching an inflection point where human conception in space is more probable to occur, and we still have vast data gaps that need to be filled on biological reproduction.  I’d advocate that the best way to go about filling those gaps would be a systematic approach using mammalian test subjects to determine safe and ethically acceptable gravity parameters for reproduction.  We already know a decent amount about the impacts of higher radiation levels on reproduction from data gathered on Earth, but with microgravity we’ve still got a long way to go, and we don’t know what the synergistic effects of microgravity and radiation are together either.  With regard to experiments, NASA researchers have actually already designed extensive mammalian reproduction experiments with university partners, but those experiments haven’t been funded by the agency.  There was a comprehensive experiment platform called MICEHAB (Multigenerational Independent Colony for Extraterrestrial Habitation, Autonomy and Behavior) that was proposed back in 2015, around the time I was completing my PhD dissertation.  It would effectively be a robot-maintained mini space station that would study the microgravity and radiation effects on rodents in spaceflight over multiple generations, which of course requires sexual reproduction.  That experiment alone would prove enormously beneficial to data collection efforts.  It would be important to study said generations and physiological impacts at variable gravity levels as you mentioned – think the Moon, Mars, 0.5 Earth G, 0.75 Earth G and so on, so we could fine tune what level of impact we as a species are medically and ethically willing to accept in order to settle new worlds.  With regard to ASRI’s experiment roadmap, our intent is to start with smaller, simpler experiments that will garner us more data on individual stages of reproduction first using live mice and rats, with the hope of eventually moving on to complex and comprehensive experiments like MICEHAB.  Once we have a good plot of data over the course of many experiments, we can hopefully move on to primate relative studies to establish safe parameters for human trials.  I anticipate the small mammal experiments alone will take at least five years were we to launch our first mission at this very moment – though speed is often dependent on level of funding, as happens with most science.

SSP: If contraceptives are recommended to prevent conception during space tourism voyages, the paper calls for validation of the efficacy of these methods in off-world environments.  Do your plans for variable gravity experiments include such studies and how would you design the protocol?

AL: Well, the first important thing to remember is that contraceptives are known to fail occasionally on Earth – condoms can break (especially if used incorrectly), and even orally-taken birth control pills aren’t considered 100% effective. Currently ASRI doesn’t have plans for contraception studies because that’s further forward than we can reasonably forecast at this point. Frankly we need to establish medical parameters first regarding conception in space and know where the risk lines are before we implement birth control studies using humans. We have to take many small steps before we get there. Once we do have established limits for safe reproduction in space environments, we would look to operate any birth control studies within those parameters to determine efficacy. That way if the contraceptives do fail, we at least know the resulting pregnancy has a reasonable chance of success.

SSP: Should experiments on mammalian reproduction in variable gravity determine that fetal developmental or health issues arise after conception and gestation in less than 1g, do you think this may lead to a significant shift in the long-term strategy for space settlement (e.g. toward O’Neill type artificial gravity space settlements) if children are to be born and raised in space?

AL:  I certainly think so.  There’s a lot at stake here.  If we can’t safely birth and grow new generations of humans at a Martian gravity level (0.38 Earth G), then we’ve largely lost Mars as a destination for permanent multigenerational settlement. Fully grown adults can live and work down on the planet itself, but we’d need to come up with an alternate nearby solution for pregnant mothers and children growing up to certain age.  From an engineering perspective, artificial gravity space settlements like an O’Neill cylinder make the most sense to me personally, so long as there’s Earth-level radiation shielding and gravity, and you can recreate Earth-like environments within those structures.  During our conversation at ISDC I referred to it as an “Orbital Incubator” concept, though I’m of course not the first person to ever discuss something like that.

SSP: I appreciate you sharing your PhD Thesis with me. In that work you developed the Reproduction and Development in Off-Earth Environments (RADIO-EE) Scale to provide a metric that could help future researchers identify potential issues/threats to human reproduction in space environments, i.e. microgravity and radiation. Respecting your request that the images of the metric not be published at this time, qualitatively, the scale plots the different phases of reproduction, fetal development, live birth and beyond against levels of gravity or radiation in outer space environments encompassing the range from microgravity all the way up to 1g (and even higher). The scale displays green, amber, and red areas mapping safe, cautionary, and forbidden zones, respectively, dependent on location (e.g. Moon, Mars, free space, etc.). When I originally read your thesis I thought you included both gravity and radiation on the same chart but after our discussions I understand that they would have to be separated out. I also acknowledge that we have no data at this time and the metric is a work in process to be filled in as experiments are performed in space. Have you considered using three dimensions (gravity on x-axis, radiation on the y-axis, viability on the z-axis) and create a surface function for viability. Does that make sense?

AL: I’m totally with you on the 3D model scale (I’ve always thought of it like navigating a “tunnel” made up of green data points to reach the end of the reproductive cycle safely).  The scale was originally envisioned as separate graphs for Microgravity/Hypergravity and Radiation, but obviously we couldn’t combine those in 2D because those two different factors can vary wildly depending on where you’re physically located in the solar system/outer space in general.  So the best answer is to effectively plot green, amber, and red “zones” on each chart (again based on location), then make sure that wherever we’re trying to grow/raise offspring (of any Earth species) we’re keeping our expectant mothers and children in double-green zones (for both gravity, and radiation).  Now the third axis would actually be time (i.e. what point are you at in the reproductive cycle), with viability being determined by where all three axes meet in a green/amber/red zone.

I’d like to thank Alex for this informative discussion and look forward to further updates as his research progresses. We urgently need his insights to inform ethical policies and practices regarding reproduction for the space tourism industry in the short term, and eventually for having and raising healthy children wherever we decide to establish space settlements. Readers can listen to Alex describe his research live and talk to him in person when he appears on The Space Show currently scheduled for August 27.

Posted on

Reproduction off Earth and its implications for space settlement

Launch of the Space Shuttle Atlantis (STS-66) on November 3, 1994. The mission carried an experiment called NIH.Rodent 1, the first of only two study’s to date on rats launched at mid-pregnancy and landed close to full term to study the effects of microgravity on reproduction. Credits: NASA

In a MDPI Journal Life paper, Alexandra Proshchina and a team* of Russian researchers summarize the research that has been performed thus far on reproduction of invertebrates in space. As mentioned in the article, the only data we have on mammalian reproduction in microgravity since the dawn of the space age is from two experiments carried out over 26 years ago. The studies looked at pregnant rats launched aboard the Space Shuttle on missions STS-66 and STS-70 in 1994 and 1995 respectively, and there have never been any births of mammals in space. This huge knowledge gap on reproduction in space is problematic for human space settlement. Yet Elon Musk, The Mars Society, and other groups are charging ahead with plans for cities on Mars. What if we discover that humans cannot have healthy babies in 0.38g? SSP has covered the quest for determining the gravity prescription before looking at JAXA’s effort to at least start experimenting with artificial gravity in space, albeit on adult mammals (mice). We are still waiting for JAXA’s published results of 1/6g experiments carried out in 2019.

The data from the Space Shuttle program only looked at part of the gestation period (after 9 days) and only in microgravity. The results did not bode well for reproduction in space. Some findings “…clearly indicate that microgravity, and possibly other nonspecific effects of spaceflight, can alter the normal development of the brain itself.”

Histological cross section through a representative rat brain from NIH.Rodent 1 experiment from STS-66. Left side (a) is low magnification and right side (b-d) are high magnification. Red arrows show areas of neurodegeneration. 1 – Nasal cavity, 2 – olfactory nerve, 3 – olfactory bulb, 4 – eye, 5 – cortex telencephali, 6 – hippocampus, 7 – fourth ventricle, 8 – cerebellum. Credits: Alexandra Proshchina et al.*

So we have this one piece of data for reproduction in microgravity and nothing in higher gravitational fields except what we know here on Earth in 1g.

Would partial gravity like on the Moon or Mars be sufficient for normal fetal development in rats (or mammals in general, especially humans) during the full gestation period? If problems are identified could it be extrapolated to human reproduction? The fact that homo sapiens and their ancestors evolved on Earth in 1g for hundreds of thousands of years is a big red flag for future space colonists that hope to settle on the surface of planetary bodies and have children.

We don’t know how lower gravity conditions could affect embryonic cell growth. How would the changes in surface tension and embryo cell adhesion be altered in these environments? We have very little data on cellular mechanisms and embryonic alterations that lower gravity may induce that could affect fetal development.

“There are also many other questions to be answered about vertebrate development under space flight conditions.”

A recent report on giving birth in space by SpaceTech Analytics looks at many of the factors that need to be considered for human reproduction off Earth. Most problems could be potentially mitigated through engineering solutions such as radiation protection, medical innovations tailored for space use, life support technology, etc. In this entire presentation the authors gave very little consideration to partial gravity affects on human embryos and child birth. One slide (number 70) out of 85 discusses these issues.

It is clear that more and longer term experiments will be necessary to determine how partial gravity affects the reproduction and development of mammals before humans settle space. Some researchers are actually considering genetic modification to allow healthy reproduction in space, and the ethical considerations associated with this course of action. Obviously, such a drastic methods would come only if there was no other alternative. One would think that building O’Neill type habitats rotating to produce 1g of artificial gravity would be preferable to such extreme measures.

Clearly, we need a space based artificial gravity laboratory to carry out ethical clinical studies on the gravity prescription for human reproduction, starting with rodents and other lower organisms. SSP recently covered a kilometer long version of such a facility that could be deployed in a single Falcon Heavy launch. And don’t forget Joe Carroll’s proposal for a LEO partial gravity test facility. Doesn’t it make sense to invest in such a facility and do the proper research before (or at least in parallel to) detailed engineering studies of colonies on the Moon or Mars intended for long term settlement? This research could inform decision making on where we will eventually establish permanent space settlements: on the surface of smaller worlds or in free space settlements envisioned by Gerard K. O’Neill. Elon Musk may want to consider such a facility before he gets too far down the road to establishing cities on Mars.

* Authors of Reproduction and the Early Development of Vertebrates in Space: Problems, Results, Opportunities: Alexandra Proshchina, Victoria Gulimova, Anastasia Kharlamova, Yuliya Krivova, Nadezhda Besova, Rustam Berdiev and Sergey Saveliev.

Posted on

Determining the gravity prescription for long term space settlement

Credits: Dai Shiba et al.* / Nature. http://creativecommons.org/licenses/by/4.0/

If humanity is to ever move off Earth, clearly we will need to be able to have children wherever we establish long term settlements. But, as humans have evolved over millions of years in Earth’s gravitational field, normal gestation may not be possible on the Moon or Mars. This is probably the most important physiological question to be answered before outposts are permanently occupied on these worlds. We can shield people from radiation, we can recycle wastes and use ISRU to replenish consumables for life support. But we may find that artificial gravity either in free space rotating habitats or on planetary surface settlements is required for settlers to have healthy children. In fact, when I asked Dr. Shawna Pandya, a physician and expert in space medicine about it on The Space Show, she said “…that is the million dollar question”.

Numerous studies have shown the deleterious effects of long term microgravity on human health. So we know that humans will need some level of gravity for sustainable occupation. But what level is enough to stave off the effects of lower gravity on human health and what about reproduction under these conditions? Plus, there is the problem of how to run ethical clinical studies to answer these questions? The Japan Aerospace Exploration Agency (JAXA) has started research in this area by studying mice under variable gravity conditions aboard their Kibo module on the International Space Station using a Multiple Artificial-gravity Research System (MARS). Results of this first ever long term space based mouse habitation study with artificial gravity were published in a paper called Development of new experimental platform ‘MARS’—Multiple Artificial-gravity Research System—to elucidate the impacts of micro/partial gravity on mice in Nature back in 2017. The authors* of the paper found that significant decreases in bone density and muscle mass of the mice reared under microgravity conditions were evident when compared to a cohort raised under 1G indicating that artificial gravity simulating the surface of the Earth may prevent negative health effects of microgravity in space. The next obvious step was to test the mice in 1/6 G simulating conditions on the Moon. This experiment was ran in 2019 but the results have not yet been published. SSP has reached out to JAXA with an inquiry on when we can expect a report. This post will be amended with an update if and when an answer is received.

Reproduction of mice or other mammals has not been studied in space under variable gravity conditions. The problem screams out for a dedicated space based artificial gravity facility such as the Space Studies Institute’s G-Lab and others (e.g. Joe Carroll’s Partial Gravity Test Facility ). Even if such a laboratory existed, how would ethical clinical studies on higher mammal animal models to simulate human physiology during pregnancy be carried out? Answering this question will come first before the million dollar one.

June 2, 2023 Update: JAXA finally released the results of their 2019 study on mice subjected to 1/6 G partial gravity in a paper in Nature in April. There is good news and not-so-good news. The good news is that 1/6 G partial gravity prevents muscle atrophy in mice. The downside is that this level of artificial gravity cannot prevent changes in muscle fiber (myofiber) and gene modification induced by microgravity. There appears to be a threshold between 1/6G and Earth-normal gravity, yet to be determined, for skeletal muscle adaptation.

______________________________

* Authors of Development of new experimental platform ‘MARS’—Multiple Artificial-gravity Research System—to elucidate the impacts of micro/partial gravity on mice: Dai Shiba, Hiroyasu Mizuno, Akane Yumoto, Michihiko Shimomura, Hiroe Kobayashi, Hironobu Morita1, Miki Shimbo, Michito Hamada, Takashi Kudo,
Masahiro Shinohara, Hiroshi Asahara, Masaki Shirakawa and Satoru Takahash

Posted on

Lunar Cruiser: JAXA and Toyota name their rover

JAXA/Toyota Lunar Cruiser. Credits: Toyota

In a recent press release, the Japan Aerospace Exploration Agency (JAXA) and Toyota announced that they will name their crewed pressurized rover “Lunar Cruiser”. There have been some updates since we initially covered this topic. For instance, work this year progressed on simulations modeling power and heat dissipation while driving and the use of virtual reality to determine the layout of equipment in the vehicles’ cabin. In addition there have been discussions among over 100 partners in various industries of a “future lunar surface-based society” in an effort to “…gather the knowledge, experience and technological capabilities of enterprises from across a variety of industries in their attempt to realize their dream of sustaining continuous activities on the surface of the moon…”

Hopefully “Team Japan”, as the consortium is called, will take into consideration mitigation of the risks caused by lunar dust in the design and use studies of the Lunar Cruiser, as discussed in my presentation at the Moon Society’s Lunar Development Conference. At some point we hope to see the Lunar Cruiser navigating the network of roads that will hopefully be constructed as proposed by the Space Development Network. It may look like this: